On latter circumstances, i hence could differentiate each other central patches and you will ?1 spot for each region of the membranous tissues ( Shape step 1 and you will Desk 1)
Into the Yards. seminigra queens, we seen one another unmarried pair and you can numerous pairs from melanized patches ( Contour 1 and you will Supplementary Profile S4 ), and then we never ever receive permanent mating plugs within this species. The latest shipment away from numerous spots ranged between step three and you will eight personalized areas, with assorted form of preparations. Regarding 33 M. seminigra specimens reviewed, 15 demonstrated single matched spots pattern, twelve shown a single matched up spots in conjunction with a main area, and you will six specimens displayed a simultaneous patch development ( Second Contour S4 ). During these agreements, new central melanized spots matched up the new inferred position of the spatha-the central build about mating connect ( Shape 2E,I). It’s worthy of bringing-up we grabbed an extra recently mated Yards. seminigra king, which was carrying 2 mating plugs: 1 is affixed in her own genital chamber, although the most other is actually onto the outside epidermis regarding this lady gut, ranging from sterna IV and you will V ( Additional Shape S4A ). That it freshly mated was not completely physogastric, and thus wasn’t utilized 
in our very own chief dataset.
Men genital medicine and vaginal sternum from M. fasciculata. Ventral view of vaginal medicine, metasomal sternum S-VIII, and you will metasomal sternum S-IX, given that located in to the virgin guys (A). Ventral look at metasomal sternum S-VIII (B) and metasomal sternum S-IX (C). Vaginal pill within the ventral take a look at, proving the new gonocoxites and you can a couple of gonostyli (D). Vaginal supplement into the dorsal consider, to provide the latest spatha and you can a couple of manhood valves (E). Vaginal capsule into the ventral see, demonstrating during the series, new rotation of the cock valves, prior to gonocoxites lateral compressing (F–H). Genital product having caused manhood regulators and you may gonocoxites compressed laterally, when you look at the dorsal (I) and you can ventral check (J).
Men vaginal product and you will vaginal sternum out-of Meters. fasciculata. Ventral view of vaginal product, metasomal sternum S-VIII, and you can metasomal sternum S-IX, due to the fact receive in to the virgin guys (A). Ventral view of metasomal sternum S-VIII (B) and you will metasomal sternum S-IX (C). Genital tablet from inside the ventral take a look at, demonstrating the fresh new gonocoxites and you may a set of gonostyli (D). Vaginal capsule when you look at the dorsal check, to provide the fresh spatha and you may a set of knob regulators (E). Genital pill inside the ventral look at, showing in the series, the new rotation of dick valves, just before gonocoxites horizontal compressing (F–H). Vaginal tablet having triggered cock valves and you can gonocoxites compressed sideways, in dorsal (I) and you can ventral see (J).
Practical morphology of one’s men vaginal supplement
The male genitalia regarding Yards. fasciculata keeps step three portion: 2 overlapping metasomal sterna (S-VIII and S-IX) and a genital tablet ( Profile dos). The newest vaginal tablet enjoys a set of nearly triangular gonocoxites, where a pair of enough time gonostyli while the pair of striking knob regulators was articulated; dorsally there clearly was a slim gonobase and you may a median spatha ( Figure 2E,I). Such sclerites is actually highly kept along with her by the a series of membranes that enable their 100 % free articulation. All portion is firmly sclerotized, apart from the newest putatively sensitive and painful setae towards the distal third out-of gonostyli; and they are positioned ventrally from the male human anatomy.
The female genitalia off Yards. fasciculata is located inside an effective chamber shaped of the tergum and sternum of your sixth intestinal segment ( Profile 3). The last part of the people person is membranous, poorly sclerotized, held by pairs from horizontal hemitergites (T-VIII and you will T-IX). Remarkably due to new membranous tail, we see a set of small setose gonostyli, a main light bulb (the fresh new gonopore town), and you may a set of narrow, horizontal rami. Between such formations together with sternum S-VI below, just below the fresh new gonopore urban area, you’ll find highest membranous purse-tissues pockets with reduced sclerotization-to that your men mating plug try attached inside mating ( Shape 3A,J) and you can copulatory marks are found ( Second Profile S5 ). Membranous purse is actually illustrated by the a white area beneath the gonopore during the Profile 3. Such membranous structures are offered within the Contour step 1 for all varieties, in virgin and mated queens, even in the event the relative dimensions and you can positioning can be looked at in more detail inside the Secondary Figure S5 .